Fig. 2.

Effects of the gem-difluorobisarylic derivatives on COX-1 and COX-2 activity. a COX-1 activity. HEK-293 cells overexpressing recombinant COX-1 were treated with 10 and 50 µM of all compounds for 45 min prior to the addition of 10 µM arachidonic acid (AA). PGE₂ was measured. b COX-2 activity. BMDM cells were treated with 10 µM of ASA for 30 min, washed, and 10 ng/mL LPS was added for 24 h to induce COX-2. Cells were further incubated with 10 and 50 µM of each compound prior to the addition of 10 µM AA. PGE₂ was measured. Data are represented as mean ± SEM (n = 4), *p < 0.05 versus AA for COX-1 activity, and versus LPS + ASA + AA for COX-2 activity (One-way Anova followed by the Dunnett’s test)