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. 2019 Oct 31;17:86. doi: 10.1186/s12915-019-0701-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 10 — GLIPR1L1 loss disrupts IZUMO1 redistribution during the acrosome reaction. a Sperm were stained for IZUMO1 localization and the acrosome was labeled with PNA (green), while DNA was stained with DAPI (blue). Scale bar = 20 μm. b The percentage of sperm that displayed IZUMO1 relocalization was scored in non-acrosome-reacted (Non-AR) and acrosome-reacted (AR) sperm from WT and Glipr1l1^−/− mice. This experiment was replicated five times with a minimum of 200 spermatozoa being examined in each. Representative images are shown. *P < 0.05, **P < 0.01, ***P < 0.001. Individual data points for each replicate are provided in Additional file 6: Raw data