Fig. 2.

IZUMO1 and GLIPR1L1 reside in multimeric protein complexes. a Native protein complexes were extracted from acrosome-reacted mouse spermatozoa and resolved by BN-PAGE. b A single lane of the BN-PAGE gel was then placed atop an SDS-PAGE gel and the individual proteins within each complex resolved according to their molecular weight. c Gels were then used for immunoblotting with IZUMO1 or GLIPR1L1 antibodies. Each of these experiments was repeated three times and representative images are shown. The boxed section indicates the position of labeled proteins vertically aligned with complexes I and IV