Fig. 4.

IZUMO1 and GLIPR1L1 localization in developing mouse germ cells. a Enriched populations of pachytene spermatocytes and b round spermatids were purified and sequentially labeled with IZUMO1 or GLIPR1L1 and appropriate Alexa Fluor 488-conjugated secondary antibodies (green) followed by Alexa Fluor 594-conjugated PNA (red). Labeling was also conducted on capacitated spermatozoa that were either c acrosome intact or d acrosome reacted. This experiment was replicated three times, and representative images are shown. Scale bar = 10 μm