Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Oct 31;17:86. doi: 10.1186/s12915-019-0701-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 5 — Co-localization of IZUMO1 and GLIPR1L1 in developing mouse germ cells using an in situ proximity ligation assay. a The cells were counterstained with DAPI (blue) and PNA (green). This experiment was replicated three times, and representative images are shown. b In the case of capacitated spermatozoa, a lower magnification image is also included to highlight the differences in PLA labeling between acrosome intact and acrosome-reacted (arrowhead) spermatozoa. Scale bar = 10 μm. c The percentage of cells displaying PLA positive labeling was recorded. Each experiment was replicated three times and the data are expressed as the mean ± S.E.M. *P < 0.05, compared with spermatocytes. Individual data points for each replicate are provided in Additional file 6: Raw data