Figure 2.

Apoptosis is induced in the ileum but not liver during the acute response to APAP intoxication. Six‐ to eight‐week‐old male C57BL/6J mice were fasted for 12 hours and treated with 500 mg/kg APAP by IP injection. Food was returned to the animals, and they were euthanized 4 hours later. Formalin‐fixed paraffin‐embedded sections of liver and distal ileum tissue were analyzed by (A) H&E staining and (B) IHC staining for cleaved caspase‐3. All provided images were taken at magnification ×200 and are representative of at least three mice per group. Scale bars represent 150 μm; arrows indicate apoptotic bodies.