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Ectopic expression of RSPO2 in HH7 cells was protumorigenic. (A) Western blots for YAP using nuclear, cytoplasmic, and whole‐cell lysates from control‐HH7 (RFP) cells and two replicates of RSPO2‐HH7 cells. Bands below YAP are nonspecific. (B) RSPO2 overexpression caused anchorage‐independent growth in soft agar. (C) Quantification of (B). Bars show mean + SD. (D) Tumors formed in the NRG xenograft model of RSPO2‐HH7 cells injected subcutaneously.
