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. 2019 Nov 1;18:151. doi: 10.1186/s12943-019-1072-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — E-cadherin is involved in circ-AKT3-dependent inhibition of ccRCC cells migration and invasion. a Protein expression levels of metastasis related genes were screened through Western blotting analysis. b-c TCGA analysis of the expression levels of E-cadherin in paired and unpaired ccRCC tissues. d The schematic flowchart shows the pipelines of miRNAs which could bond to circ-AKT3 and E-cadherin 3′-UTR via online bioinformatic network. e-f the RT-PCR and qRT-PCR arrays determined that miR-296-3p, miR-382-5p and miR-326 could interact with circ-AKT3 via RNA pull down assay