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. 2019 Nov 1;11:53. doi: 10.1186/s13099-019-0335-4

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — EMSA of the binding of RstA. EMSA of the binding of RstA to P_LEE1 (a), P_LEE2/3 (b), P_LEE4 (c), and P_LEE5 (d), rpoS (e, negative control), P_asr (f), P_hdeA (g) and P_yeaI (h). PCR products were added to the reaction mixtures at 40 ng each. RstA protein was added to the reaction buffer in each assay in lanes 2–5 at 0.25, 0.5, 1, and 2 μM, respectively. No protein was added in lane 1. No binding was observed in a–e, while binding was observed in f–h