Figure 4.

Gibberellins dynamics underlie differential seed germination in WOSR accessions. (A) Levels of all GA metabolites differentially increase in WOSR accessions during seed germination. Stacked columns histograms show the distribution of GA metabolites (GA₅₁, GA₃₄, GA₂₉, GA₂₄, GA₂₀, GA₈, GA₄) in the varieties during seed germination. (B), (C), and (D) Active gibberellin GA₄ and GA₂₄ accumulation was markedly delayed in C032 accession from 36 to 72 hai compared to C129 and C033, and GA₈ content is higher in C129 accession compared to C033 and C032, correlating with their germination speed. Stacked columns histograms show the relative accumulation patterns of GA₄ (active GA), GA₂₄ (GA₄ precursor), and GA₈ inactive GAs in C129, C033, and C032 accessions, respectively. Dashed curves highlight the GA₄ (active GA) accumulation dynamics in each accession. All data were obtained using three pooled biological replicates (50 seeds per replicate). For complete value data set, see Supplementary Table S1 (Datasheet 3).