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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 Apr 12;30(6):919–931. doi: 10.1007/s13361-019-02159-w

Figure 3.

Figure 3

Cross section distributions of [subP+3H]3+ (left), [subP+2H]2+ (middle), and [subP(3- 11)+2H]2+ (right) and corresponding ions of Pro→Ala analogues, for the purpose of assigning Xxx-Pro peptide bonds as cis or trans. For [subP(3–11)+2H]2+, the top spectrum (A) shows the cross section distribution of [subP(3–11)(P4A)+2H]2+ ions corresponding to subP(3–11)(P4A) produced from incubation of subP(P4A), the middle spectrum (B) shows the cross section distribution of [subP(3–11)(P4A)+2H]2+ from the synthesized subP(3–11)(P4A) peptide, and the bottom spectrum (C) shows the mobility profile of [subP(3–11)+2H]2+ produced during incubation of subP in ethanol at 55 °C after 150 min.