Figure 4. Rictor^Lyz2-Cre mice are more susceptible to DSS-induced acute colitis.

(A–F) Rictor^fl/fl and Rictor^Lyz2-Cre mice were challenged with 5% DSS in drinking water for 5 days, followed by normal water for 9 (A) or 3 (B–F) days. Survival (A) and weight (B) was monitored daily. One representative of 2 independent experiments is shown. (A) Data represent survival of 15 Rictor^fl/fl and 11 Rictor^Lyz2-Cre mice. P value was determined by log-rank test. (B) Data represent the mean ± SEM (n = 9–11). (C) Colon length on day 8. Data represent the mean ± SEM (n = 9–11). (D) Colon permeability on day 8 by FITC-Dextran gavage and subsequent measurement of fluorescence intensity in the serum. Data represent the mean ± SEM (n = 9–10). (E) Representative dot plots for the leukocyte population (CD45⁺) of Rictor^fl/fl and Rictor^Lyz2-Cre lamina propria cells are shown. (F) Lamina propria cells of the colon were isolated on day 8 and flow cytometric analysis of immune cell populations was performed (Supplemental Figure 2). Data represent the mean ± SEM (n = 12). P values for B–D and F were determined by unpaired 2-tailed Student’s t test. *P < 0.05; **P < 0.01.