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. 2019 Oct 17;4(20):e131712. doi: 10.1172/jci.insight.131712

Figure 9. Distinct and overlapping expression patterns for Tgr5, Mrgpra3, and Mrgprc11 in colon-innervating DRG neurons from CVH mice.

Figure 9

(A) Single-cell RT-PCR of 46 retrogradely traced colon-innervating DRG neurons from 4 CVH mice reveals that subpopulations express Tgr5 (20%), Mrgpra3 (39%), Mrgprc11 (74%), Trpv1 (65%), and Trpa1 (74%). (B) Donut plot showing expression and coexpression of genes encoding Tgr5, Mrgpra3, Mrgprc11, Trpv1, and Trpa1 in 46 individual retrogradely traced colon-innervating DRG neurons from CVH mice. Each color represents an individual gene, with expression marked by bold shading (Tgr5, outer ring; Trpa1, inner ring). Some CVH DRG neurons express all targets, while other neurons express combinations of targets. (C–E) Group data showing (C) Tgr5, (D) Mrgpra3, and (E) Mrgprc11 are expressed individually within subpopulations of colon-innervating DRG neurons and also coexpressed together in other subpopulations. For example, of the Tgr5 expressing colon-innervating DRG neurons from CVH mice, 56% coexpress Mrgpra3, and 33% coexpress Mrgprc11. Tgr5, Mrgpra3, and Mrgprc11 also heavily coexpress with Trpv1 and, in particular, Trpa1 in CVH states.