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. 2019 Oct 17;4(20):e130521. doi: 10.1172/jci.insight.130521

Figure 3. Schematic timeline representing study design for lentiviral-mediated microRNA manipulation in the VMH and hypoglycemic clamps.

Figure 3

(A) Male Sprague-Dawley rats were subjected to either artificial cerebrospinal fluid (aCSF) or lentivirus (containing either control or targeted microRNA sequence) injection into the VMH by using a stereotaxic frame. Three weeks following intra-VMH injection, all rats subsequently had vascular catheters implanted under ketamine anesthesia. Four days following vascular surgery, rats that received aCSF into the VMH were subjected to either RS or RH for 3 days, while rats that received lentiviruses into the VMH were subjected to the RH only. Following overnight fasting (i.e., after 4 weeks of intra-VMH injection), all rats were subjected to the hyperinsulinemic (20 mU/kg/min) hypoglycemic (40–50 mg/dL for 90 minutes) clamps for assessing counterregulatory hormonal response in plasma. (B and C) Blood glucose levels (mg/dL) during the 3-day protocol in RS rats treated with intra-VMH aCSF-RS and RH rats treated with either aCSF-RH or control lentivirus (lenti-7a-cont-RH or lenti-665-cont-RH) or microRNA-encoding lentivirus (lenti-miR-7a-RH or lenti-miR-665-RH). Data are expressed as mean ± SEM (n = 6–7/group).