Skip to main content
. 2019 Oct 25;10:2508. doi: 10.3389/fimmu.2019.02508

Figure 2.

Figure 2

Heat-killed pathogens do not directly induce EC activation. (A) Mean RNA expression level (VST) of 213 DE genes responding to either LPS, S. pneumoniae or C. albicans on EC. Color scales by VST value, ranging from weak to strong expression (6–16). Data are represented as mean of three replications. (B) Number of shared and unique genes induced in EC upon stimulation. (C) List of 16 common DE genes (with protein names), and their pathway enriched by Reactome. (D,E) Protein expression levels of E-selectin, VCAM-1, ICAM-1, IL-6, and IL-8 in EC at 6 and 24 h after stimulation with LPS, S. pneumoniae (SPneu) and C. albicans (C.alb), respectively. E-selectin, VCAM-1 and ICAM-1 were measured by flow cytometry, data were presented as Geometric Mean Florescence Intensity (MFI). IL-6 and IL-8 were measured by ELISA, data were presented as pg/ml. Data are shown as mean (SD), representative for three independent experiments. ****p < 0.0001.