Figure 3.

Leukocyte-mediators significantly induce EC activation. (A–C) Protein levels of E-selectin, VCAM-1, and ICAM-1 measured by flow cytometry, and secreted IL-6 and IL-8 measured by ELISA on EC after 6 h exposed to direct stimulation (first 2 columns) and to PBMC medium (other columns). Data were presented as Mean florescence Intensity (MFI) for E-selectin, VCAM-1 and ICAM-1 and pg/ml for IL-6 and IL-8. For IL-6 and IL-8, the amount of cytokines presented in PBMC medium before adding to EC were plotted in the 3rd and 4th column, whereas the amount of total cytokines after exposure to EC were plotted in the 5th and 6th column. Colors represent three different individuals of whom PBMC were isolated. (D) RNA expression levels (VST) of 255 DE genes in EC induced by PBMC medium. (E) The number of shared and unique DE genes between various conditions. (F) Top five pathways enriched by 60 common genes induced by all PBMC medium. (G–I) Top 5 pathways enriched by genes responded to (G) Spneu_Sup and Calb_Sup, (H) only Calb_Sup, and (I) only Spneu_Sup. Data are shown as (A–C) representative of three independent experiments (mean and SD) (D,E) mean from three biological replications (F–I), –log₁₀ of q-value. **p < 0.01, ***p < 0.001, ****p < 0.0001.