Table 4.

Separation of function variants that affect posttranslational modifications

Variant	Modification defects	Localization changes	Steady-state abundance	Lipid phosphatase activity	Impacted cellular phenotypes	References
K289Ea	Ø ubiquitination	↓ Nucleus	Normal	Normal	↓ G1/S transition delay	Han et al. 2000; Trotman et al. 2007; Song et al. 2011; Matreyek et al. 2018
K254Ra	Ø sumoylation	↓ Nucleus	Normal	↓	↓ DNA damage repair	Huang et al. 2012; Bassi et al. 2013; Vuono et al. 2016; Matreyek et al. 2018
Y240Fa	Ø phosphorylation	↓ Nucleus	Normal	↓ Koul et al. (2002) Normal Nathanson et al. (2012)	↓ DNA damage repair	Koul et al. 2002; Nathanson et al. 2012; Matreyek et al. 2018; Ma et al. 2019
T366A/S370A	Ø phosphorylation	None reported	↓	↑	↓ Cell growth	Vazquez et al. 2000; Al-Khouri et al. 2005; Odriozola et al. 2007
A4b	Ø phosphorylation	↓ Plasma membrane ↑ Nucleus	↓	↑	↓ Cell growth	Vazquez et al. 2000, 2006; Das et al. 2003; Rahdar et al. 2009; Nguyen et al. 2015
K266R	Ø sumoylation	↓ Nucleus	Normal	↓↓	NS	Huang et al. 2012

NS, not studied.

^aSentinel variant.

^bA4, S380A/T382A/T383A/S385A.