Skip to main content
. 2019 Nov 1;33(21-22):1591–1612. doi: 10.1101/gad.328971.119

Figure 5.

Figure 5.

Binding sites of the dosage compensation complex are shuffled along the X chromosome in D. melanogaster, D. busckii, and D. virilis. (A) Immunostaining of male polytene chromosomes with MOF antibody (green) in D. melanogaster, D. busckii, and D. virilis. DNA is counterstained with Hoechst (blue). Scale bars, 20 µm. Immunostaining of female polytene chromosomes are shown in Supplemental Figure S7. (B) Position (gray vertical bars) and obs/exp Hi-C contacts (red arcs) between high-confidence roX2 sites (HAS) along the entire X chromosome in D. melanogaster, D. busckii, and D. virilis. (C) Example of one corresponding HAS as indicated by the red arrows in B. Coverage of roX2 ChIRP-seq reads, H4K16ac ChIP-seq reads from separated female and male third-instar larvae, SBs, BUSCOs, and genes annotated in D. melanogaster, highlighting RpL7A and dx, two genes with phenotypic classes related to viability reduction corresponding, respectively, to the BUSCOs EOG09150ATD and EOG091502A5 in all three species. (D) Example gene that moved between an autosome and the X chromosome when comparing D. melanogaster and D. virilis. MED20 is localized on chromosome 2L in D. melanogaster but on chromosome X in D. virilis (Dvir GJ18844). The surrounding genes on this SB on chromosome 2L maintained the same order (see corresponding BUSCOs numbered from 1 to 7 and gene track). MED20 in D. virilis (Dvir GJ18844) is localized on chromosome X in between two surrounding SBs, within a H4K16ac domain (male). Two additional examples are shown in Supplemental Figure S7B,C.