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. 2019 Nov 1;33(21-22):1539–1554. doi: 10.1101/gad.328682.119

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© 2019 Morafraile et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 1. — The replication checkpoint inhibits origin firing genome-wide. (A) Replication profile of the indicated strains after release from alpha factor into 200 mM HU for 90 min. Copy number (y-axis) was derived by normalizing the sequencing reads at 90 min to the reads at 0 min (G1). Annotated origins (top) are colored according to their average time of replication in an unperturbed S-phase (T_rep) from early (red) to late (green). Only chromosome XI is shown for simplicity. Unconfirmed origins also fire in the sld3-A dbf4-A strain, examples of which are indicated by the *. The telomeres are excluded due to mappability issues. (B) Rad53 western blot from the indicated strains released from G1 phase arrest with alpha factor (0 min) into 200 mM HU for the indicated time points. (C) Graph of number of origins from A that fired in at least 20% of cells. (D) Box plot of each origin from C plotted according to the distance to its nearest neighboring fired origin. (E,F) As in A, except the strains were released from G1 phase into 0.02% MMS for 90 min (E) and 180 min (F).