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. 2019 Nov 1;33(21-22):1539–1554. doi: 10.1101/gad.328682.119

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© 2019 Morafraile et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 2. — Checkpoint inhibition of origin firing prevents the accumulation of DNA damage markers. (A) Western blots from the indicated strains released from G1 phase arrest with alpha factor (0 min) into 200 mM HU for the indicated time points. (B) Quantification of Rad52-GFP foci in the indicated strains released from G1 phase arrest with alpha factor (0 min) into 200 mM HU for the indicated time points. Error bars are SD, n = 7. (C) As in B, except strains were released from G1 phase (0 min) into either 0.02% MMS or into medium in the absence of drug for the indicated time points. Error bars are SD, n = 5. (D) Quantification of Rad52-GFP foci in the indicated strains released from G1 phase arrest with alpha factor (0 min) into 0.02% MMS for 90 min. Error bars are SD, n = 3.