Figure 6.

eGFP reporter genes driven by the exemplar promoters are regulated by Integrator. (A) The promoter and 5′ UTR of each of the indicated protein-coding genes was cloned upstream of an eGFP reporter. The plasmids were then individually transfected into DL1 cells that had been treated with the indicated dsRNAs. CuSO₄ was added for the last 14 h only when measuring eGFP production from the MtnA promoter. Northern blots were used to quantify expression of each eGFP reporter mRNA. Representative blots are shown. Loading controls and quantification are provided in Supplemental Figure S10. (B) DL1 cells were treated with the indicated dsRNAs and then transfected with a reporter plasmid that produces eGFP when the encoded U4:39B snRNA fails to be properly processed at its 3′ end. Northern blots were used to quantify eGFP mRNA expression that is a result of U4:39B readthrough. A representative blot is shown. Loading controls and quantification are provided in Supplemental Figure S10.