Biochemical evaluation of gba1, gba2, and double gba1:gba2 KO zebrafish larvae. A: ABP labeling of homogenate of individual zebrafish larvae at 5 dpf (WT, gba1−/−, gba2−/−, and gba1−/−:gba2−/−, n = 3) with ABP 1 (top panel) or ABP 2 (middle panel). In lane –, the sample is denatured prior to ABP addition; CBB staining and β-actin were used as loading control (lower panels). B: GlcSph, HexCer, GlcCer, and GlcChol levels were determined of individual 5 dpf zebrafish larvae in picomoles per fish; WT (n = 15), gba1−/− (n = 15), gba2−/− (n = 12), and gba1−/−:gba2−/− (n = 13) for GlcSph, HexCer, and GlcChol; and WT (n = 9), gba1−/− (n = 9), gba2−/− (n = 9), and gba1−/−:gba2−/− (n = 10) for GlcCer. Data are depicted as mean ± SD and analyzed by one-way ANOVA (Dunnett’s test) with WT as control group. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. C: Developing zebrafish embryos were harvested at different ages and GlcSph, HexCer, GlcCer, and GlcChol levels were determined in picomoles per fish [8 h post fertilization (n = 4–5), 1 dpf (n = 3–5), 2 dpf (n = 3–5), 3 dpf (n = 3–5), 4 dpf (n = 3–6)]. Data on relevant lipid levels of 5 dpf larvae are obtained from C. Data are depicted as mean ± SEM