Fig. 5.

Chemical inactivation of Gba1 shows full gba1 deficiency with increased GlcSph levels. WT, gba2^−/−, gba1^−/−, and gba1^−/−:gba2^−/− embryos were treated with vehicle [0.1% (v/v) DMSO] or inhibitor 3 (10 μM) for 5 days and relevant lipid levels were determined of individual larvae in picomoles per fish (n = 7-15) (A) or active β-glucosidase enzyme was visualized with ABP 1 (top panel) or ABP 2 (middle panel) (B); CBB staining was used as loading control (lower panel). C: Gba1^−/− embryos were harvested at different ages and active Gba1 was visualized with ABP 1. Data of GlcSph, HexCer, and GlcChol are depicted as mean ± SD and analyzed using one-way ANOVA (Tukey’s test). *P < 0.05, **P < 0.001, ***P < 0.001, and ****P < 0.0001.