Fig. 2.

Albumin and ApoM protein expression level in the mouse plasma. (A) Plasma (1 μl) from each genotype of the mice was separated by SDS-PAGE. Albumin was revealed by Coomassie Brilliant Blue staining, and ApoM was detected by Western blot analysis by using anti-ApoM antibody (Abcam, #EPR2904). B: Plasma concentration of albumin in WT and ApoM-KO mice was determined using LBISTM Albumin Mouse ELISA kit (FUJIFILM Wako Pure Chemical Corp.) according to the manufacturer’s instructions. Data represent mean ± SD (n = 3). C: Plasma expression level of ApoM in WT and Alb-KO was assessed by Western blot analysis. The intensity of each band was quantified by using ImageJ software (National Institutes of Health). Data represent mean ± SD (n = 3; *P < 0.01, by Student’s t-test). The figure shows representative data from two independent experiments with basically the same results.