Fig. 6.

Sinomenine (SIN) and cisplatin regulated the PI3K/AKT/Wnt signaling pathways in gastric cancer. HGC-27 cell lines were treated with SIN and/or cisplatin for 24 hours as above. (a) Effects of SIN and cisplatin on the PI3L/AKT pathway were analyzed by Western blotting. (b) Analysis of the Bcl-2/Bax ratio in (a). (c) Analysis of the cleaved caspase 3/procaspase 3 ratio in (a). (d) Effects of SIN and cisplatin on the Wnt/β-catenin pathways were analyzed by Western blotting. (e) Quantitative analysis of proteins in (d). (f) Immunofluorescence staining of β-catenin expression in HGC-27 cells. B-catenin proteins were stained with rhodamine (red) and nuclei were stained with DAPI (blue). All the experiments are carried out three times.*P < 0.05 versus control cells; #P < 0.05 versus 1 μg/mL cisplatin alone cells.