Table 1. Proteins up- or down-regulated in ceh-60 mutants versus wild type.
| Down-regulated (mutant versus wild type) | |||||
| Protein ID | Protein | Gene name | Fold change | p-value | Fold change RNAseq [12] |
| VIT5_CAEEL | Vitellogenin 5 | vit-5 | 0.15 | <0.0001 | 0.035 |
| VIT4_CAEEL | Vitellogenin 4 | vit-4 | 0.16 | <0.0001 | 0.012 |
| VIT3_CAEEL | Vitellogenin 3 | vit-3 | 0.16 | <0.0001 | 0.048 |
| VIT2_CAEEL | Vitellogenin 2 | vit-2 | 0.20 | <0.0001 | 0.161 |
| VIT6_CAEEL | Vitellogenin 6 | vit-6 | 0.22 | <0.0001 | 0.053 |
| VIT1_CAEEL | Vitellogenin 1 | vit-1 | 0.27 | <0.0001 | 0.109 |
| Q19813_CAEEL | Collagen-120 | col-120 | 0.56 | <0.0001 | - |
| CALM_CAEEL | Calmodulin | cmd-1 | 0.56 | <0.0001 | - |
| G5EEH6_CAEEL | Isovaleryl-CoA dehydrogenase | ivd-1 | 0.66 | 0.0008 | - |
| PSMD3_CAEEL | 26S proteasome non-ATPase regulatory subunit 3 | rpn-3 | 0.68 | 0.0023 | - |
| Q8MNT7_CAEEL | Hydroxyacyl-coA hydrolase | hach-1 | 0.69 | 0.005 | - |
| ULE4_CAEEL | UPF0375 protein | ule-4 | 0.72 | 0.0156 | 0.760 |
| Q8MXT6_CAEEL | Collagen-106 | col-106 | 0.73 | 0.0201 | 1.433 |
| O17685_CAEEL | Uncharacterized protein | C49F5.7 | 0.73 | 0.0201 | - |
| O44145_CAEEL | Permeable eggshell | perm-2 | 0.74 | 0.0277 | - |
| C2BR91_CAEEL | Cystatin | C39B5.5 | 0.74 | 0.028 | 2.443 |
| H2KZG6_CAEEL | Acyl-CoA dehydrogenase | acdh-1 | 0.75 | 0.028 | - |
| Up-regulated (mutant versus wild type) | |||||
| Protein ID | Protein | Gene name | Fold change | p-value | Fold change RNAseq [12] |
| PGP1_CAEEL | Multidrug resistance protein 1 | pgp-1 | 1.94 | <0.0001 | 3.870 |
| O45444_CAEEL | C-type lectin 63 | clec-63 | 1.54 | 0.0005 | 3.522 |
| O18307_CAEEL | Uncharacterized protein | ZK909.3 | 1.48 | 0.0021 | - |
| RB11A_CAEEL | Ras-related protein Rab11.1 | rab-11.1 | 1.44 | 0.0052 | - |
| O76367_CAEEL | Cytochrome Oxidase assembly protein | cox-6c | 1.38 | 0.0201 | 1.214 |
| Q17475_CAEEL | Uncharacterized protein | B0334.3 | 1.38 | 0.0197 | 1.473 |
| O17621_CAEEL | Uncharacterized protein | C29F7.2 | 1.35 | 0.0277 | 2.849 |
| L8E833_CAEEL | Uncharacterized protein | W05H9.1 | 1.34 | 0.028 | 1.911 |
| Q18577_CAEEL | Uncharacterized protein | C42D4.1 | 1.32 | 0.0367 | 1.402 |
| GST7_CAEEL | Probable glutathione S-transferase 7 | gst-7 | 1.3 | 0.0395 | 1.168 |
| Q22562_CAEEL | Uncharacterized protein | T19B10.2 | 1.3 | 0.0395 | 1.479 |
| P4HA1_CAEEL | Prolyl 4-hydroxylase subunit alpha-1 | dpy-18 | 1.29 | 0.0395 | - |
| IFB2_CAEEL | Intermediate filament protein B2 | ifb-2 | 1.27 | 0.0395 | 1.900 |
| PDIA4_CAEEL | Probable protein disulfide-isomerase A4 | C14B9.2 | 1.26 | 0.0395 | 1.915 |
We identified respectively 17 and 14 differentially down- and up-regulated proteins (fold changes <0.75 or >1.25) in ceh-60(lst466). The 6 VITs are the most down-regulated. Cuticle collagens (COL-120 and COL-106) and fatty acid metabolism proteins (IVD-1, HACH-1, and ACDH-1) represent other down-regulated proteins. Two known immune defense-related proteins (PGP-1, CLEC-63) are up-regulated. RNAseq fold changes from ceh-60(ok1485) versus control [12] are shown for comparison. Transcripts for which no significant change was reported in the RNAseq dataset (p < 0.01) are marked with “-.”
Abbreviation: CoA, coenzyme A.