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. 2019 Oct 18;98(42):e17594. doi: 10.1097/MD.0000000000017594

Figure 2.

Figure 2

BMPR2 expression and enhanced BMP and TGF-β signaling. (A) Western blot analysis of BMPR2, phosphorylated Smad1/5/8, and Smad2/3, Erk1/2, and p38 MAP kinase. PBMCs were cultured with BMP2 (200 ng/mL), TGF-β (20 ng/mL), and PMA (100 nM) for 24 h to stimulate Smad signaling. C stands for control and P for patient. (B) RNA expression of BMPR2, the BMP target genes, ID1, SMAD6, and STAT1, and the TGF-β-target genes, Atf4, Gadd45b, Emp1, and Myc. PBMCs were cultured in α-MEM-based complete medium for 6 h and harvested for the real-time qPCR. ∗P < .05 patient versus controls. BMPR2 = bone morphogenetic protein receptor II, PBMCs = peripheral blood mononuclear cells, qPCR = quantitative polymerase chain reaction, TGF-β = transforming growth factor-β.