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The sequence of direct and reverse primers of FGFR1 and GAPDH genes
| Reverse primer (5’…3’) |
Direct primer (5’…3’) |
Gene |
|---|---|---|
| TGTCACCAGCCTTCATGACC | ATGGTTGCAGGTGATGTGGT | FGFR1 |
| TTCAGCTCAGGGATGACCTT | CCACCCAGAAGACTGTGGAT | GAPDH |
Fusion curve: concerning the usage of SYBR Green I in our study, a fusion curve was drawn for both FGFR1 and GAPDH genes. As seen, this curve has only two peaks, one for FGFR1 and the other for GAPHD. So there is no nonspecific product in our test.
