Figure 4. Microscopic wetness forming with natural leaf washes.
(A) ivy leaf wash. (B) orange leaf wash. In both leaf washes, droplet formation around microbiota cells including fungi, yeast, and bacteria can be observed. Leaf wash protocols and drying conditions are described in Materials and methods.
Figure 4—figure supplement 1. Microscopic surface wetness forming with natural leaf washes.
A few diH20 drops were loaded onto the abaxial surface of an ivy leaf, and left for 2 hr at room temperature. A volume of 340 µl was aspired with a pipette, transferred to our standard surface drying platform, and left to dry using our described protocol (28°C, 85% RH, see Materials and methods). The plate surface was imaged ~5 hr after macroscopic drying of the well.