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Multicycle smFISH of gpa-3 within the same worm using ICP-enhanced smFISH, and puncta were identified using FISH-quant. (a) Individual puncta were resolved after initial hybridization. (b) After DNase I treatment for 4 h, probes were dehybridized and removed by the ICP-based ion pump. (c) The same puncta were resolved again after rehybridization enhanced by ICP. Insets show the magnified area at the vicinity of the target mRNA molecule.
