Figure 5.

Glycocalyx crowding with spectator glycoconjugates differentially affects the association of ConA and SNA with endogenous glycans depending on their distribution throughout the glycocalyx. The glycopolymer spectators inhibit both the initial rates and saturation binding of ConA to the less accessible mannose structures. Glycocalyx crowding influences the association of SNA with sialic acid residues only at later time points near saturation binding, once the more available peripheral glycans have become occupied.