Table 1.

Dissociation constants, K_d, determined by fluorescence data and Eq. 1 for each binding event between ConA and immobilized mannose at different mole fractions, χ. Errors are reported as one standard deviation from the mean taking from three different features in the array.

	[ConA] nM
	4800	2400	960	480	240	96	48	9.6
χ	Kd (nM)
1	270 ± 50	180 ± 42	240 ± 54	18 ± 2	110 ± 78	52 ± 5	43 ± 15	NA
0.9	270 ± 37	4 ± 1	350 ± 66	41 ± 15	100 ± 49	49 ± 11	28 ± 5	NA
0.8	710 ± 150	490 ± 110	400 ± 77	88 ± 26	130 ± 6	56 ± 14	29 ± 6	NA
0.7	910 ± 256	650 ± 190	420 ± 91	91 ± 39	140 ± 34	71 ± 8	37 ± 3	NA
0.6	380 ± 68	550 ± 200	460 ± 170	140 ± 57	160 ± 44	74 ± 31	39 ± 8	NA
0.5	2100 ± 426	1100 ± 290	570 ± 120	170 ± 100	170 ± 33	64 ± 14	51 ± 11	NA
0.4	1800 ±370	1100 ± 100	660 ± 140	190 ± 61	180 ± 7	74 ± 6	66 ± 3	NA
0.3	2200 ± 490	1100 ± 340	550 ± 78	190 ± 85	200 ± 37	76 ± 10	70 ± 5	NA
0.2	NB	NB	NB	NB	NB	NB	NB	NB
0.1	NB	NB	NB	NB	NB	NB	NB	NB
0.01	NB	NB	NB	NB	NB	NB	NB	NB

NB = No binding NA = Features undetectable