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. Author manuscript; available in PMC: 2020 Feb 28.
Published in final edited form as: Mucosal Immunol. 2019 Aug 28;12(6):1382–1390. doi: 10.1038/s41385-019-0196-7

Figure 4. WT and Tollip−/− Macrophage Cytokine Responses after L. pneumophila (Lp) Infection in vitro.

Figure 4.

Bone marrow-derived macrophages were isolated and infected with either negative control (mock), WT L. pneumophila (WT Lp), flagellin-deficient L. pneumophila (ΔflaA Lp), or dot-icm-locus-deficient L. pneumophila (ΔdotA Lp) on the JR32 background at MOI 10 overnight and A) TNF, B) IL-1β, and C) IL-6 concentrations were measured from cellular supernatants. * p < 0.05, Student’s two-sided t-test. Experiment shown is representative of three independent experiments, each performed in triplicate. Error bars represent +/− SEM of mean.