Figure 1.

Generation of a mouse‐human chimeric anti‐coxsackievirus and adenovirus receptor (CAR) mAb, ch6G10A. A, Flow cytometric analysis. DU‐145 human prostate cancer cells were incubated with 10 μg/mL ch6G10A, a mouse‐human chimeric anti‐CAR mAb and subjected to flow cytometric analysis. B, Assessment of antibody‐dependent cellular cytotoxicity activity. DU‐145 cells were incubated with human natural killer cell line KHYG‐1/Fcγ receptor IIIA in the presence of the indicated antibodies at 100 μg/mL for 4 h. Results are expressed as means ± SD of three independent experiments carried out in triplicate. Statistical analysis was done by Student's t test (**P < .01 vs control). C, Assessment of complement‐dependent cytotoxicity activity. DU‐145 cells were incubated with 5% rabbit complement in the presence of the indicated antibodies at 10 μg/mL for 4 h. Results are expressed as means ± SD of three independent experiments carried out in triplicate. Statistical analysis was done by Student's t test (*P < .05 vs control)