Figure 1.

Knockdown of octamer transcription factor 1 (OCT1) reduces proliferation and migration of 22Rv1 cells. A, Comparison of androgen receptor (AR) and AR‐V7 expression in castration‐resistant prostate cancer (CRPC) model cells, 22Rv1, with hormone naïve prostate cancer cells, LNCaP. Cells were treated with 10 nmol/L dihydrotestosterone (DHT) or vehicle for 24 h. Western blot analysis for AR and AR‐V7 expression was carried out and β‐actin was used as a loading control. B, Difference of androgen‐independent and ‐dependent cell growth in 22Rv1 cells from LNCaP cells. Cells were treated with 10 nmol/L DHT or vehicle. MTS assay was done at the indicated time points (N = 4). Results are presented as mean and SD. **P < 0.01, ***P < 0.001. C, Validation of siRNA targeting for OCT1. 22Rv1 cells were transfected with 10 nmol/L siControl or 10 nmol/L siOCT1 (#A and #B) for 48 h and then treated with 10 nmol/L DHT or vehicle for 24 h. Western blot analysis for OCT1 expression was carried out and β‐actin was used as a loading control. D, Knockdown of OCT1 inhibits CRPC cell proliferation. 22Rv1 cells were treated with 10 nmol/L siControl or 10 nmol/L siOCT1 (#A and #B). MTS assay was carried out at the indicated time points (N = 6). Results are presented as mean and SD. *P < 0.05, ***P < 0.001. E, Knockdown of OCT1 inhibits CRPC cell migration. 22Rv1 cells were treated with siControl or siOCT1 #A or #B. After 48 h incubation, cell migration assay was done (N = 5). Results are presented as mean and SD. ***P < 0.001. Left panels show representative views of migrated cells (siControl and siOCT1 #A and #B) in 22Rv1 cells