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. 2019 Sep 16;110(11):3476–3485. doi: 10.1111/cas.14183

Figure 3.

Figure 3

Transcriptional regulatory mechanism of four novel octamer transcription factor 1 (OCT1)‐target genes. A, ChIP‐seq analysis of OCT1 and androgen receptor (AR) binding in the enhancer/promoter regions of disks large‐associated protein 5 (DLGAP5), kinesin family member 15 (KIF15), non‐SMC condensin I complex subunit G (NCAPG), and NDC80 kinetochore complex component (NUF2) in 22Rv1 cells are shown. Arrows indicate the direction of each gene. B, Four OCT1‐target genes were regulated by AR. 22Rv1 cells were treated with 5 nmol/L siControl (siCont.) or siAR (#A and #B) for 48 h and then treated with 10 nmol/L dihydrotestosterone (DHT) or vehicle for 24 h. mRNA expression level of each gene was measured by qRT‐PCR. Results are presented as mean and SD (N = 3). *P < 0.05, **P < 0.01, ***P < 0.001, compared with cells treated with siControl