Figure 2.

Highly expressed NSUN2 promotes NOZ cell proliferation and tumorigenesis in vitro. A, mRNA level of NSUN2 in gall bladder cancer (GBC) cell lines NOZ, GBC‐SD, SGC‐996, EHGB‐1, OCUG‐1 and the human gallbladder epithelium cell line HGEpC. B, Left panel shows the protein level of NSUN2 in GBC cell lines and the human gallbladder epithelium cell line. γ‐actin was used as the loading control. The right panel shows the quantitative results. C and F, Western blot analysis of the efficiency of NSUN2 knockdown or overexpression in NOZ cells. γ‐actin was used as the loading control. * indicates non‐specific bands and the red arrow represents Flag‐NSUN2. D and G, The relative cell growth rates were determined by CCK‐8 proliferation assays at various time points in NOZ cells. E and H, Representative images of colony formation with NSUN2 knockdown or overexpression induced by RNAi or plasmid transfection in NOZ cells. The numbers of colonies are presented in the right panel. *P < 0.05, **P < 0.01 and ***P < 0.001