Figure 4.

Erythropoietin‐producing hepatocellular carcinoma receptor A6 (EPHA6) physically interacts with bone morphogenetic protein (BMP) type I receptor ALK‐2. A, Immunoblots showing coimmunoprecipitation of ALK‐2 with EPHA6. TGS‐03 and TGS‐04 cells overexpressing LacZ, wild‐type EPHA6, and constitutively active ALK‐2 (A2‐CA) were immunoprecipitated (IP) with anti‐FLAG antibody 1 day after adenovirus transduction, followed by HA and FLAG immunoblotting for ALK‐2 and EPHA6, respectively. TCL, total cell lysate; pSMAD1/5, phospho‐SMAD1/5. B, Immunoblots of TGS‐03 and TGS‐04 cells after immunoprecipitation of ALK‐2‐CA protein using anti‐HA antibody and the corresponding TCL with the same experimental setting as in (A). C, Immunoblot analysis of TGS‐03 cells showing coimmunoprecipitation of EPHA6 with HA‐tagged ALK‐2‐CA treated with or without 1 μmol/L LDN193189 for 1 d. LacZ overexpression serves as a negative control for all experiments in Figure 4