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. 2019 Sep 20;110(11):3486–3496. doi: 10.1111/cas.14187

Figure 5.

Figure 5

Wild‐type and kinase‐dead erythropoietin‐producing hepatocellular carcinoma receptor A6 (EPHA6) function similarly to promote bone morphogenetic protein (BMP)‐2‐induced apoptosis in TGS‐01 cells. A, FACS analysis of TGS‐01 cells infected with ad‐LacZ, ad‐EPHA6‐WT and ad‐EPHA6‐KD treated or untreated with BMP‐2 for 7 d. Percentages represent apoptotic cells. Propidium iodide (PI) signal is plotted on Y‐axis and Annexin‐V signal on X‐axis. B‐D, TGS‐01 cells were infected with ad‐LacZ, ad‐EPHA6‐WT, and ad‐EPHA6‐KD in the presence or absence of BMP‐2 for 24 h and RNA‐sequencing analysis was carried out. Genes with fragments per kilobase of exon per million mapped fragments (FPKM) values equal or higher than 3 are selected for gene set enrichment analysis. Plots show enrichment of apoptotic genes in LacZ cells treated with vs without BMP‐2 treatment (B), EPHA6‐WT vs LacZ‐expressing cells (both treated with BMP‐2) (C), and EPHA6‐KD vs LacZ‐expressing cells (both treated with BMP‐2) (D). NES, normalized enrichment score