Figure 4.

Stem cell properties endowed by E‐cadherin‐Fc chimera protein (E‐cad‐Fc). A, Sphere formation of each group seeded at a density of 1000 cells per well cultured for 18 days. In the Degron(+) E‐cad‐Fc(+) group, cells were cultured with E‐cad‐Fc–coated plates for 48 h and then transferred to ultra‐low attachment plates. Spheroid morphology is shown in whole view (left) and magnified view (right). Rough‐edged spheres are marked by black arrows, and smooth spheres by white arrowheads. Scale bar, 200 μm. B‐a, Sphere size ≥100 μm in diameter was counted to assess sphere‐formation ability. *P < .05. B‐b, Sphere size ≥100 μm in diameter was counted to assess sphere‐formation ability, and smooth‐edged spheres and rough‐edged spheres were separately counted. *P < .05, **P < .01. C‐a, Proliferation assay and chemoresistance assay. In the Degron(+) E‐cad‐Fc(+) group, cells were cultured with E‐cad‐Fc–coated plates for 48 h and then transferred to plates at a density of 5000 cells per well, and cultured with DMEM supplemented with 10% FBS. Proliferation was measured by CCK‐8 every 24 h. C‐b, Cells of each group were treated with fluorouracil (5‐FU) or oxaliplatin (L‐OHP) for 72 h. After drug treatment, cell survival rates were measured. *P < .05