Fig. 3.

First division restitution (FDR) of meiosis in a microspore-derived haploid (n = 21, ABR) from a triticale hybrid: a column of meiocytes with typical signs of FDR, i.e., univalents positioned on the metaphase I plate, and separating sister chromatids in anaphase (live-stained material); b a close-up view of meiocytes in metaphase I with an equatorial and a polar view of the metaphase plate; c a meiocyte in anaphase I, where all chromosomes at the pole clearly comprise sister chromatids; d stages from metaphase I (four meiocytes) to various stages of anaphase I, showing clear sister chromatid separation and the presence of laggard chromosomes (LC) (marked with an arrow); e dyads at the final stage of division, where similar nuclei sizes are observed in each dyad, along with a rare micronucleus (MIC) (marked with an arrow) putatively resulting from laggard chromosomes; f the sectorial nature of FDR—a portion of the meiocyte column is shown where the meiocyte marked with an arrow is undergoing “normal” meiotic division (NM), while the remaining meiocytes show division with restitution; and g the final products of meiotic division: three tetrads and three dyads. Scale bar 40 μm