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. 2019 Nov 1;9:15867. doi: 10.1038/s41598-019-52416-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mean rank of expression stability of eight candidate reference genes in F. hepatica. A number (from 1 to 8) was assigned to each stability coefficient obtained from four algorithms. The mean rank with SEM is shown for (A) the analysis of three intra-mammalian stages (NEJs, immature and adult flukes), and (B) the analysis of in vitro-cultured juveniles (NEJs before and after 28 days culture). Genes were ranked from the least stable (on the left) to the most stable (on the right).