Table 1.
Overview of candidate reference genes for the study of gene expression in F. hepatica.
| Gene name | Gene ID | Homology (e-value)* | Protein function |
|---|---|---|---|
| Fhtbcd | maker-scaffold10x_815_pilon-snap-gene-1.87 | Tubulin-specific chaperone D [H. sapiens, NP_005984.3] (7e-177) | Cofactor D is one of four proteins involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. |
| Fheprs | maker-scaffold10x_14_pilon-snap-gene-0.109 | Glutamyl-prolyl-tRNA synthetase [H. sapiens, NP_004437.2] (0.0) | The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyses the aminoacylation of glutamic acid and proline tRNA species. |
| Fhletm1 | maker-scaffold10x_721_pilon-snap-gene-0.10 |
Leucine zipper and EF-hand containing transmembrane protein 1 [Homo sapiens, NP_036450.1] (2e-85) |
The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. |
| Fhactb | augustus_masked-scaffold10x_269_pilon-processed-gene-0.18 | Actin, cytoplasmic 1 [H. sapiens, NP_001092.1] (0.0) | Actins are highly conserved proteins that are involved in cell motility, structure, integrity and intracellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two non-muscle cytoskeletal actins that are ubiquitously expressed. |
| Fhsnrpa1 | maker-scaffold10x_234_pilon-snap-gene-0.20 | U2 small nuclear ribonucleoprotein A’ [H. sapiens, NP_003081.2] (5e-72) | This gene encodes a protein which is a component of the spliceosome and it is involved in pre-mRNA splicing. |
| Fhppp1cb | maker-scaffold10x_238_pilon-snap-gene-0.95 | Protein phosphatase 1 catalytic subunit beta [H. sapiens, NP_002700.1] (0.0) | The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes. |
| Fhpsmb7 | maker-scaffold10x_1452_pilon-augustus-gene-0.11 | Proteasome subunit beta type-7 [H. sapiens, NP_002790.1] (1e-79) | Important component of the cellular protein degradation complex. |
| Fhgapdh | maker-scaffold10x_2706_pilon-snap-gene-0.15 | Glyceraldehyde-3-phosphate dehydrogenase [H. sapiens, NP_001276674.1] (0.0) | GAPDH catalyzes the sixth step of the glycolysis by converting D-glyceraldehyde 3-phosphate to 3-phospho-D-glyceroyl phosphate. |
*Determined by NCBI BLAST.