Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 1;10:5014. doi: 10.1038/s41467-019-12897-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — ERG controls the expression of multiple regulators of coagulation in a tissue-specific manner. a qPCR screen analysis of genes involved in the regulation of coagulation in HUVEC was performed at 48 h after ERG inhibition by siRNA (n = 4–6 independent experiments). Data were normalised to GAPDH. b, c qPCR analysis of selected targets genes directly or indirectly involved in coagulation and endothelial markers in whole liver (b) and lung (c) lysates from adult control (Erg^fl/fl) and ERG-deficient (Erg^iEC-KO) mice (n = 4–5 per genotype). Data were normalised to 18S. d–e qPCR analysis of thrombomodulin (TM) mRNA expression in whole liver (d) and lung (e) lysates from adult Erg^fl/fl and Erg^iEC-KO mice (n = 5 per genotype). Data were normalised to 18S. f Immunoblot and quantification of WB for TM in protein extracts of lung samples from adult Erg^fl/fl and Erg^iEC-KO mice 30 days after tamoxifen injection (n = 3 mice per group). Data were normalized to GAPDH. All graphical data are mean ± s.e.m, *P < 0.05, Student’s t-test. Source data are provided as a Source Data file