Fig. 3.

The cyanide insensitive terminal oxidase Cio mediates self-protection of cooperators. a Competition between WT PAO1 (black), CioA-null cooperator (red), or PA4133-null cooperator (blue) and LasR-null mutants. The initial frequency of cheaters was 0.01. Five biological replicates were performed. Lines represent best-fit polynomial regressions for each condition. b Expression of lasB, lasR and rhlR in WT PAO1 (black), CioA-null mutant (red), and PA4133-null mutant (blue). c Elastase production by WT PAO1 (black), CioA-null mutant (red), and PA4133-null mutant (blue). For b, c means, s.e.m., and individual biological replicates are shown (n = 3)