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. 2019 Nov 1;9:15850. doi: 10.1038/s41598-019-52063-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-PAGE profiles of skim milk samples before trypsinisation for shotgun analysis. Pos: culture-positive samples. Neg: culture-negative samples. SAU: milk samples positive for Staphylococcus aureus. NAS: milk samples positive for non-aureus staphylococci. M: molecular weight markers. Sample numbers correspond to those listed in Table 1. Molecular weight references are indicated on the left. Proteins with a molecular weight corresponding to the main electrophoretic bands are indicated on the right as a reference. One microliter of skim milk was loaded in each lane.