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. 2019 Nov 1;9:15850. doi: 10.1038/s41598-019-52063-2

Table 3.

Significantly differential proteins in Staphylococcus-positive milk with RSC ≥ 1.5 or RSC ≤ −1.5.

Accession Description RSC
Pos/Neg		 RSC
SAU/Neg		 RSC
NAS/Neg		 General
function
P48616 Vimentin* 4.78 4.96 4.58 S
A0A0A7NSG7 Cathelicidin 4* 4.75 4.72 4.78 I
Q1JPB0 Leukocyte elastase inhibitor* 4.09 4.17 4 I
X5I0G0 Cathelicidin 1* 4.04 4.1 3.98 I
C8CF42 Probactenecin 7* 3.91 4.02 3.78 I
P46193 Annexin A1 3.86 4.02 3.67 I
P52176 Matrix metalloproteinase-9* 3.8 3.93 3.66 I
P54228 Cathelicidin 6* 3.67 3.72 3.62 I
A0A0A7V3V9 Cathelicidin 2* 3.64 3.74 3.52 I
E1AHZ7 Protein S100A8* 3.52 3.54 3.51 I
Q92176 Coronin-1A* 3.51 3.51 3.51 I
A5D7D1 Alpha-actinin-4 3.48 3.63 3.31 S
P62803 Histone H4* 3.47 3.67 3.24 S
Q2KJD0 Tubulin beta-5 chain 3.45 3.63 3.25 S
Q0VCM4 Glycogen phosphorylase 3.42 3.43 3.42 CM
Q3MHM5 Tubulin beta-4B chain 3.37 3.49 3.23 S
Q3T149 Heat shock protein beta-1* 3.33 3.39 3.26 PD
Q27991 Myosin-10* 3.31 3.28 3.34 S
L0L830 Cathelicidin 7* 3.26 3.37 3.15 I
P40673 High mobility group protein B2* 3.25 3.43 3.04 I
Q5MAR3 Integrin beta* 3.22 3.48 2.9 I
Q0VCW4 L-serine dehydratase/L-threonine deaminase* 3.09 3.16 3.01 AM
P55052 Fatty acid-binding protein, epidermal* 3.08 3.08 3.08 LM
P02253 Histone H1.2* 3.08 3.06 3.11 S
F6MFD5 Peptidoglycan-recognition protein 3.08 3.26 2.86 I
P62808 Histone H2B type 1 3.04 3.04 3.04 S
Q9TU25 Ras-related C3 botulinum toxin substrate 2 2.97 2.99 2.95 I
Q9TU03 Rho GDP-dissociation inhibitor 2* 2.97 3.13 2.79 S
Q2TBL6 Transaldolase* 2.96 3.05 2.86 CM
V9LY96 Cathelicidin 5* 2.95 3.05 2.84 I
Q2HJ86 Tubulin alpha-1D chain 2.95 3.11 2.76 S
Q3SWX7 Annexin A3* 2.86 3.04 2.65 I
Q3B7N2 Alpha-actinin-1 2.85 3.01 2.68 S
Q5E9F5 Transgelin-2* 2.85 2.83 2.86 S
Q3SYV4 Adenylyl cyclase-associated protein 1 2.84 2.78 2.9 S
Q2KJH4 WD repeat-containing protein 1* 2.81 2.63 2.97 S
P68432 Histone H3.1* 2.79 2.91 2.66 S
A7E3Q8 Plastin-3* 2.79 2.86 2.7 S
P81948 Tubulin alpha-4A chain 2.74 2.93 2.51 S
Q3MHR7 Actin-related protein 2/3 complex subunit 2* 2.68 2.61 2.75 S
A7MB62 Actin-related protein 2 2.62 2.6 2.64 S
Q5E9B1 L-lactate dehydrogenase B chain 2.61 2.69 2.52 CM
M1JVB9 Haptoglobin 2.59 2.68 2.49 I
A0A0C5AGQ3 Lysozyme* 2.59 2.64 2.55 I
Q6B855 Transketolase 2.59 2.65 2.54 CM
P02584 Profilin-1 2.58 2.6 2.56 S
Q5E956 Triosephosphate isomerase 2.53 2.56 2.49 S
P10103 High mobility group protein B1 2.52 2.73 2.28 I
A2I7N1 Serpin A3-5 2.52 2.67 2.35 PD
Q3ZBD7 Glucose-6-phosphate isomerase 2.5 2.59 2.4 CM
P0C0S9 Histone H2A type 1 2.5 2.54 2.45 S
Q3T0P6 Phosphoglycerate kinase 1 2.5 2.53 2.47 CM
P19858 L-lactate dehydrogenase A chain 2.48 2.43 2.52 CM
Q3T114 Ribonuclease UK114* 2.47 2.4 2.53 CP
Q32LE5 Isoaspartyl peptidase/L-asparaginase* 2.46 2.34 2.58 CP
P30922 Chitinase-3-like protein 1 2.45 2.32 2.57 I
Q2HJ60 Heterogeneous nuclear ribonucleoproteins A2/B1* 2.39 2.66 2.06 NM
A5A4L2 Histone H2B*,a 2.38 2.59 I
P79136 F-actin-capping protein subunit beta 2.32 2.41 2.23 S
P61157 Actin-related protein 3 2.31 2.27 2.35 S
Q5KR47 Tropomyosin alpha-3 chain 2.31 2.37 2.24 S
P09867 Heterogeneous nuclear ribonucleoprotein A1* 2.3 2.45 2.14 S
A4IF97 Myosin regulatory light chain 12B* 2.27 2.39 2.14 S
A3KMV5 Ubiquitin-like modifier-activating enzyme 1* 2.26 2.57 1.87 PD
Q2HJ57 Coactosin-like protein* 2.22 2.24 2.21 I
Q58CQ2 Actin-related protein 2/3 complex subunit 1B* 2.2 2.22 2.18 S
G3N131 Histone H1.1* 2.19 S
P49951 Clathrin heavy chain 1* 2.08 2.22 1.92 S
A5D7A0 EF-hand domain-containing protein D2* 2.08 2.2 1.95 S
Q2TA49 Vasodilator-stimulated phosphoprotein 2.06 2.42 1.58 S
Q3ZC84 Cytosolic non-specific dipeptidase 2.05 2.18 1.91 OM
P10096 Glyceraldehyde-3-phosphate dehydrogenase 2.05 2.14 1.97 CM
P60661 Myosin light polypeptide 6 2.02 2.15 1.87 S
Q3T0D0 Heterogeneous nuclear ribonucleoprotein K* 2.01 2.08 1.94 NM
P33433 Histidine-rich glycoproteina 2.01 2.52 I
Q4U5R3 Proteasome activator complex subunit 1 2.01 2.07 1.96 PD
Q32PI5 Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform 1.98 2.29 1.59 S
Q56JZ9 Glia maturation factor gamma* 1.97 2.02 1.92 S
Q0VCX1 Complement C1s subcomponent* 1.96 2.24 1.62 I
Q3SZH7 Leukotriene A-4 hydrolase* 1.96 1.91 2.02 I
P31081 60 kDa heat shock protein, mitochondrial* 1.95 2.12 1.76 I
Q32LP0 Fermitin family homolog 3* 1.94 2.15 1.69 I
D2U6Q0 Serum amyloid A protein* 1.93 1.86 2 I
P62326 Thymosin beta-4* 1.91 2.03 1.78 I
P13752 BOLA class I histocompatibility antigen, alpha chain BL3-6* 1.9 2.08 1.7 I
Q2KJI3 Protein FAM49B* 1.89 1.87 1.92 I
Q762I5 Resistin* 1.89 1.92 1.85 LM
Q76LV2 Heat shock protein HSP 90-alpha 1.88 2.06 1.68 PD
Q3SZ54 Eukaryotic initiation factor 4A-I 1.85 1.82 1.88 GE
Q3MHL4 Adenosylhomocysteinase 1.83 1.82 1.84 AM
P80724 Brain acid soluble protein 1* 1.82 1.61 2 GE
P60712 Actin, cytoplasmic 1 1.8 2.01 1.57 S
J9Q6V1 Glutathione peroxidase* 1.8 2.05 1.5 OM
P55859 Purine nucleoside phosphorylase* 1.78 1.51 2 NM
Q9XSJ4 Alpha-enolase 1.77 1.8 1.75 CM
Q27970 Calpain-1 catalytic subunita 1.77 2.13 PD
Q865V6 Macrophage-capping protein* 1.76 1.65 1.86 S
Q9GMB8 Serine–tRNA ligase, cytoplasmica 1.74 2.02 AM
Q9BGI1 Peroxiredoxin-5, mitochondrial 1.73 1.92 1.52 OM
P51122 Acidic leucine-rich nuclear phosphoprotein 32 family member Aa 1.72 CT
Q148J6 Actin-related protein 2/3 complex subunit 4* 1.7 1.71 1.69 S
A4FUA8 F-actin-capping protein subunit alpha-1* 1.68 1.63 1.73 S
P06868 Plasminogen 1.68 1.77 1.58 C
P07589 Fibronectin 1.67 I
P13753 BOLA class I histocompatibility antigen, alpha chain BL3-7*,a 1.65 1.96 I
O46522 Cytochrome b-245 heavy chaina 1.64 2 I
Q3SZI4 14-3-3 protein theta 1.62 1.69 1.55 GE
A6H742 Plastin-1* 1.62 1.54 1.69 S
D2U6Z5 Ceruloplasminb 1.61 1.75 IM
Q3T035 Actin-related protein 2/3 complex subunit 3*,a 1.57 1.71 S
P20000 Aldehyde dehydrogenase, mitochondrial*,a 1.57 1.71 CM
P01030 Complement C4b 1.57 1.72 I
P0C0S4 Histone H2A.Za 1.56 1.66 S
Q27996 Lysozyme C, tracheal isozyme 1.56 1.51 1.61 I
Q5E9B7 Chloride intracellular channel protein 1 1.55 1.52 1.57 C
P68138 Actin, alpha skeletal musclea 1.54 1.72 S
X2IZ01 Signal transducer and activator of transcriptiona 1.54 1.59 I
Q3ZBT1 Transitional endoplasmic reticulum ATPasea 1.54 1.68 CT
Q2HJG5 Vacuolar protein sorting-associated protein 35a 1.54 1.84 I
O97680 Thioredoxina 1.53 1.79 OM
P19483 ATP synthase subunit alpha, mitochondrialb 1.52 CT
P02676 Fibrinogen beta chaina 1.79 C
P02672 Fibrinogen alpha chaina 1.83 I
Q5E9R3 EH domain-containing protein 1a 1.78 CaM
P00829 ATP synthase subunit beta, mitochondriala 1.66 OM
P81644 Apolipoprotein A-IIa 1.64 LM
Q3B7M5 LIM and SH3 domain protein 1a 1.61 S
A6H7G2 Drebrin-like proteina 1.58 I
Q3ZBH0 T-complex protein 1 subunit betaa 1.57 PD
Q3MHL7 T-complex protein 1 subunit zetaa 1.52 PD
Q7SIH1 Alpha-2-macroglobulina 1.52 I
P27214 Annexin A11a 1.52 S
Q3SZV7 Hemopexina 1.5 IM
P11116 Galectin-1b 1.61 I
P35466 Protein S100-A4b 1.6 I
O02675 Dihydropyrimidinase-related protein 2*,b 1.51 S
Q3T054 GTP-binding nuclear protein Ranb 1.61 CT
Q3T0D7 GTP-binding protein SAR1a −1.51 CT
Q71SP7 Fatty acid synthasea −1.52 −1.8 LM
P02638 Protein S100-B −1.53 I
P10790 Fatty acid-binding protein, hearta −1.53 LM
M9WP41 BTN1A1a −1.57 S
Q17QE5 Calcium and integrin-binding protein 1a −1.56 −1.85 I
Q9N0T1 Stanniocalcin-1a −1.57 −1.9 CaM
A0A0C5GDU2 Myostatina −1.59 S
Q6RUS0 Butyrophilinb −1.6 −1.75 LM
G1AQP3 Xanthine dehydrogenase/oxidasea −1.61 −1.81 LM
E9NV81 ATP-binding cassette sub-family G (WHITE) member 2a −1.62 −1.95 LM
Q05927 5′-nucleotidaseb −1.65 −1.88 NM
M4QG36 Insulin induced protein 1a −1.65 −1.88 S
O77588 Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a −1.68 −2.19 S
A5PK13 Volume-regulated anion channel subunit LRRC8C −1.68 LM
Q2KIY5 Putative phospholipase B-like 2a −1.69 −2.24 LM
P81127 Gamma-soluble NSF attachment protein −1.71 CT
Q58CY6 Prostamide/prostaglandin F synthaseb −1.73 −1.83 LM
Q06805 Tyrosine-protein kinase receptor Tie-1 −1.73 A
P37980 Inorganic pyrophosphatase −1.74 −1.99 −1.53 LM
Q3T0Q2 Transmembrane protein 59 −1.75 −1.87 −1.63 CT
Q3SYV1 Transmembrane protein 263 −1.77 CT
Q3SYS6 Calcineurin B homologous protein 1 −1.81 −1.87 −1.75 CaM
Q58DD4 Syndecan-2a −1.81 −2.13 S
D3K0R6 Plasma membrane calcium-transporting ATPase 4 −1.93 −2.04 −1.82 CaM
C1KGU3 Solute carrier family 11 member 2 −1.93 −2.02 −1.85 CT
Q5GJ77 Glycerol-3-phosphate acyltransferase 1, mitochondrial −2.01 −2.13 −1.91 LM
A0A088F8E5 Acyl-CoA synthetase short-chain family member 2 −2.08 −2.19 −1.97 LM
P08239 Guanine nucleotide-binding protein G(o) subunit alpha −2.14 −2.25 −2.03 CT
P08169 Cation-independent mannose-6-phosphate receptor −2.15 −2.47 −1.88 I
Q3ZBE9 Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating −2.17 −2.65 −1.8 LM
Q2YDI9 Ferritin, mitochondrial −2.28 −2.4 −2.17 IM
Q58DW6 Ras-related protein Rab-25 −2.28 −2.39 −2.17 CR
Q3MHW6 Monocarboxylate transporter 1 −2.48 −2.55 −2.41 CM
A0A097P9M4 Long-chain fatty acid-CoA ligase 1 −2.6 −3.2 −2.17 LM
P84466 Lanosterol synthase −2.71 −2.83 −2.61 LM
Q3MHX6 Protein OS-9a −1.57 S
Q2TBX4 Heat shock 70 kDa protein 13a −1.59 PD
Q0VD19 Sphingomyelin phosphodiesterasea −1.72 LM
Q1RMU3 Prolyl 4-hydroxylase subunit alpha-1a −1.73 S
Q5EA88 Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmica −1.8 CM
Q0IIG7 Ras-related protein Rab-5Aa −2.11 CT
A6QR11 Protein kinase C-binding protein NELL2a −2.37 CaM
Q5E9B5 Actin, gamma-enteric smooth muscleb −3.76 S

The table reports the differential proteins obtained when considering all staphylococcus-positive milk samples (Pos/Neg), only SAU-positive milk samples (SAU/Neg) or only NAS-positive milk samples (NAS/Neg). The general functional classification is indicated as follows: A, angiogenesis; AM: aminoacid metabolism; CaM, calcium metabolism; CM, carbohydrate metabolism; CP: catabolic process; CT, cellular transport; C, coagulation; GE: gene expression; I, immunity; IM, iron metabolism; LM: lipid metabolism; NM: nucleotide metabolism; OM: oxidative metabolism; PD, protein degradation; S, structure. Gene ontology results are detailed in Supplementary File 1.

*Detected in staphylococcus-positive milk (≥2 peptide spectrum matches, PSM) and not detected in healthy milk. aSignificantly changed only in SAU-positive milk. bSignificantly changed only in NAS-positive milk.