Fig. 5.

LG268 reduces the expression of FOXP3 in CD4 T cells and modulates ratios of CD8 T-cell populations in vitro. a CD4 T cells were isolated from a spleen of a wild-type mouse using negative magnetic beads. CD4 T cells were plated with anti-CD3, anti-CD28, IL2 and TGFβ for 24 h prior to adding LG268 or bexarotene for 4 days. CD4 cells where collected and levels of FOXP3 were determined by PCR. b CD3 cells were isolated with negative magnetic beads from the spleen of a wild-type mouse. CD3 T cells were stimulated with anti-CD3 and treated with LG268 for 3 days. Activation of CD4 and CD8 was evaluated by flow cytometry. Cells were stained with surface markers to identify different cell populations; Naïve: CD3⁺, CD8⁺, CD44⁻, CD62L⁺; Central memory: CD3⁺, CD8⁺, CD44⁺, CD62L⁺; Effector/effector memory: CD3⁺, CD8⁺, CD44⁺, CD62L⁻. n = 3–4, Bars represent average and error bars the standard error of the mean (SEM)