Fig. 8.

LG268 treatment prolongs survival in PyMT mice. a PyMT mice with tumor(s) 32–64 mm³ in size were treated with control diet or diet containing LG268 (100 mg/kg diet). Mice were kept on treatment until tumor burden reached IACUC-defined sizes (n = 12 mice/group). b Tumors were measured twice weekly with a caliper; total tumor volume was graphed over time. c Levels of cleaved caspase 3 and CD8 were determined by immunohistochemistry. Representative pictures for each group are shown. Scale bar represents 60 μm. d Levels of p-STAT1 and PD-L1 in tumor extract at the end point. Levels of the indicated proteins were determined by western blot. Western blot for four mice per cohort are represented. n = 12, bars represent average and error bars the standard error of the mean (SEM)