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. 2019 Nov 1;9:15848. doi: 10.1038/s41598-019-52174-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effectiveness of dinaciclib treatment on CYP3A4 and PXR expression levels. Dinaciclib dose response and time response studies. (A) A dose response study was conducted where Huh7 cells were treated with different dinaciclib concentrations for 24 hrs. The fold changes in expression levels of CYP3A4 and PXR are shown. (B) Comparison of the effect of 4-week confluency versus dinaciclib treatment on the basal expression levels of CYP3A4 and PXR. In 4-week confluent cultures, CYP3A4 and PXR levels were quantified via quantitative real-time PCR and plotted as fold expression normalized to freshly plated Huh7s. The other group was freshly plated and treated with 20 nM dinaciclib for 24 hrs. CYP3A4 and PXR expression levels were quantified in the same way. (C) A time response study was conducted where Huh7s were treated with 20 nM dinaciclib for 24 hrs. Following the treatment, the fold-changes in CYP3A4 and PXR mRNA levels were quantified via quantitative real-time PCR at different time points, up to 48 hrs. The data are presented as mean ± SEM, N = 6.